Coding

Part:BBa_K1405006

Designed by: Xuan Wang,Feng Jing,Ningze Sun   Group: iGEM14_BNU-China   (2014-10-12)

INPN+YFP

Introduction

we found that using INP derivatives containing only N-domain (INPN) as display system successfully is possible in the literature research. The passenger proteins are also various, such as Japanese Encephalitis Virus Pathogenicity, phosphate-binding protein and so on. There is also literature which alleges that since full-length INP is quite large (1,200–1,500 amino acid residues), functional truncated INP molecules may serve as better anchoring motifs to carry large heterologous proteins. Because INP is outer membrane protein, we have no idea whether INP is located on menbrane successfully. So we want to fuse INP and YFP. We,lat least, can know INP is expressed or not by observing light of YFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1882
    Illegal SapI.rc site found at 238


[edit]
Categories
Parameters
None